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2D & Isoelectric Focusing

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lEF and 2D electrophoresis

Equipped with rehydration and focusing trays.

The IEF system has been optimised to perform first-dimension isoelectric focusing (IEF) with IPG (immobilised pH gradient) strips quickly, easily and reproducibly. It can also be used with precast IEF Gels. An ideal entry-level system for both experienced and occasional IEF users, the unit is versatile enough to meet the needs of laboratories with increased throughput requirements as well as first time users.

High capacity

Its high-capacity focusing tray accommodates up to twelve IPG strips. Adjustable ‘pick-and-place’ electrodes clip conveniently anywhere within the focusing tray to resolve IPG strips 7-24cm in length and are colourcoded to prevent polarity reversal. The Electrode frame clips directly on to the cooling plate and includes adjustable electrodes to run horizontal precast IEF and PAGE gels. A cooling plate, manufactured from a special grade ceramic in a large 26x26cm surface area, facilitates effective heat dissipation and control, particularly during high voltage IEF techniques. An optional, but recommended, recirculating chiller connects quickly and easily to the cooling plate to maintain optimal operating temperatures for IPG strips and precast gels.

Rehydration

The Rehydration tray allows convenient transfer of IPG strips to the focusing tray without time-consuming removal of residual rehydration buffer and also enables the focusing tray to remain permanently in use for IEF to maximise throughput and provides useful storage at -20°C for focused strips before second-dimension runs. For those requiring a power supply, the Consort EV3330, 3000V, 300mA, 300W enables desired Volt-hours for focusing to be attained faster at maximum voltage.

Specifications

Max. commercial strip length accommodated 24cm
Max. gel dimensions on cooling plate 26x26cm
Unit dimensions (w x d x h) 55x35x10cm
Focusing tray strip capacity 12x 18 and 24cm strips; 24x 7 and 11cm strips
Operating temperature 4-25°C
Regulatory certification CE, EN61010

Features

  • A high-capacity focusing tray that accommodates up to twelve IPG strips
  • Adjustable pick-and-place electrodes clip conveniently anywhere within the focusing tray to resolve IPG strips 7-24cm in length; colour-coded to prevent polarity reversal
  • A cooling plate, manufactured from a special grade ceramic in a large 26x26cm surface area, facilitates effective heat dissipation and homogenous thermal control, particularly during high voltage IEF techniques
  • An optional, but recommended, Cleaver Scientific recirculating chiller connects quickly and easily to the cooling plate via snap-lock connectors to maintain optimal operating temperatures for IPG strips (20) and precast gels (4)
  • Rehydration tray allows convenient transfer of IPG strips to the focusing tray without time-consuming removal of residual rehydration buffer; also enables focusing tray to remain permanently in use for IEF to maximise throughput, and provides useful storage at -20 for focused strips before second-dimension runs
  • Electrode frame clips directly on to the cooling plate and includes adjustable electrodes to run horizontal precast IEF and PAGE gels