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ProtoCOL 3

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Automatic colony counting and zone measurement

The next generation instrument for colony counting, zone measurements, (inhibition and AST), membranes and a range of other applications.

ProtoCOL 3 has a unique 3 channel LED lighting system configured for exceptional illumination of all sample types. High definition, colour images taken with a 1.4 mega pixel scientific grade CCD camera ensure that even the smallest colony can be seen and counted, while zone measurements are fast, accurate and reproducible.

ProtoCOL 3 HD, the newest addition to the range, has an ultra-HD 5 megapixel camera which captures high definition images and measures zones with >99.9% accuracy, making the ProtoCOL 3 HD a must-have for vaccine potency testing and other laboratories wanting to optimize zone reading accuracy.

The ProtoCOL 3 system is available with a mounted touchscreen processor which enables ease of control while the easy-to-use software produces highly accurate results. Alternatively, the ProtoCOL 3 can now be used with a new or existing stand-alone desktop or laptop computer via USB connection – no additional PCI cards are required.

The ProtoCOL 3 comes inclusive with both colony counting and inhibition zone measuring ability. To extend its range of applications, inexpensive additional modules can be added to the software for:

  • Spiral
  • OPKA
  • SBA
  • Multi-sector
  • Multi-well
  • Ames
  • Antibiotic susceptibility testing (eAST).

Results can be automatically transferred to Excel or a LIMS system. The system can also be used with 1D barcodes. All data generated is GLP/GMP/21 CFR Part 11 compliant with a full audit trail and can be used to produce professional reports. Each system comes complete with 2 validation plates. Additional application-specific validation plates are available on request.

Specifications

ProtoCOL 3/ProtoCOL 3 Plus ProtoCOL 3 HD/ProtoCOL 3 HD Plus
Design Ergonomic housing constructed in high density foam
Integral CCD camera and lens
ProtoCOL 3 Plus comes with a mounted all-in-one PC
Ergonomic housing constructed in high density foam
Integral CCD camera and lens
ProtoCOL 3 HD Plus comes with a mounted all-in-one PC
Light shield 2 sliding doors to prevent excessive ambient light 2 sliding doors to prevent excessive ambient light
Camera Integrated, sensitive 1.4 megapixels with f1.2 lens Integrated, high definition 5 megapixels with f1.5 lens
Resolution For standard 150mm Petri dish, smallest detectable colony is 43 microns For standard 150mm Petri dish, smallest detectable colony is 43 microns
Imaging 3 channel capture for colour images 3 channel camera for colour images
Lighting Unique 3 channel (red, green, blue) LED lighting
Multi-array LED lighting (computer controlled)
Lower lighting with upper reflective lighting for all applications
Unique 3 channel (red, green, blue) LED lighting
Multi-array LED lighting (computer controlled)
Lower lighting with upper reflective lighting for all applications
External connections VESA mounted, small form factor (Mini-ITX) PC with i3 (Skylake) 2.7GHz processor
4GB Ram
500GB hard drive
Lan, WiFi and Windows 10
17″ touchscreen monitor featuring 10 touch points and PCAP technology. Due to its glass overlay, it guarantees high durability, scratch-resistance and perfect picture performance
VESA mounted, small form factor (Mini-ITX) PC with i3 (Skylake) 2.7GHz processor
4GB Ram
500GB hard drive
Lan, WiFi and Windows 10
17″ touchscreen monitor featuring 10 touch points and PCAP technology. Due to its glass overlay, it guarantees high durability, scratch-resistance and perfect picture performance
Count modes Separation of touching colonies, exclusion areas, colour mode, shape mode, size mode Separation of touching colonies, exclusion areas, colour mode, shape mode, size mode
Software Win7/10 compatible Win7/10 compatible
Database SQL database stores all data and image locations SQL database stores all data and image locations
Dimensions 450mm (h) x 500mm (d) x 400mm (w)
700mm (h) x 500mm (d) x 400mm (w)
450mm (h) x 500mm (d) x 400mm (w)
700mm (h) x 500mm (d) x 400mm (w)

Wide range
of applications

The system can be configured for a wide range of plate types. These include:

  • Pour plates
  • Spiral plates
  • Multi-well
  • SBA (serum bactericidal assay)
  • OPKA (opsonophagocytic killing assay)
  • Ames
  • Inhibition zones
  • AST (antibiotic susceptibility testing)
  • SRD (single radial immunodiffusion)
  • Chromogenic
  • Multi-sector
  • MIC
  • Dilution series

ProtoCOL 3 is being used at a world renowned vaccine research facility for testing and designing new and existing vaccines against serious bacterial diseases. One of the ways scientists there test the efficacy of vaccines is to mix serum from vaccinated participants with bacteria in an SBA assay.